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irisin inhibitor rgds  (MedChemExpress)
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MedChemExpress irisin inhibitor rgds
Irisin down-regulates PIAS4 to repress EHMT2 SUMOylation, thereby promoting osteogenic differentiation. MSCs were infected with lentivirus containing oe-NC or oe-PIAS4 with or without irisin together. A Osteogenic differentiation potential was evaluated by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). B Western blotting analysis of protein levels of PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1. (C) Cells were lysed and immunoprecipitated with EHMT2 antibody and then immunoblotted with SUMO2/3 or EHMT2 antibody. MSCs were treated with irisin together with or without <t>irisin</t> <t>inhibitor</t> <t>RGDS.</t> (D) Osteogenic differentiation potential was determined by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). (E) The protein levels of FAK, p-FAK, SKP2, PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1 were assessed by Western blotting. (F) Cell lysates were immunoprecipitated with EHMT2 antibody, followed by immunoblotting with SUMO2/3 or EHMT2 antibody. (G) Ubiquitin level of EHMT2 was analyzed by Co-IP. n = 3. One-way ANOVA (for B, E) was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001
Irisin Inhibitor Rgds, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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irisin powder  (MedChemExpress)
93
MedChemExpress irisin powder
Irisin down-regulates PIAS4 to repress EHMT2 SUMOylation, thereby promoting osteogenic differentiation. MSCs were infected with lentivirus containing oe-NC or oe-PIAS4 with or without irisin together. A Osteogenic differentiation potential was evaluated by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). B Western blotting analysis of protein levels of PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1. (C) Cells were lysed and immunoprecipitated with EHMT2 antibody and then immunoblotted with SUMO2/3 or EHMT2 antibody. MSCs were treated with irisin together with or without <t>irisin</t> <t>inhibitor</t> <t>RGDS.</t> (D) Osteogenic differentiation potential was determined by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). (E) The protein levels of FAK, p-FAK, SKP2, PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1 were assessed by Western blotting. (F) Cell lysates were immunoprecipitated with EHMT2 antibody, followed by immunoblotting with SUMO2/3 or EHMT2 antibody. (G) Ubiquitin level of EHMT2 was analyzed by Co-IP. n = 3. One-way ANOVA (for B, E) was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001
Irisin Powder, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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irisin concentrations  (BioVendor Instruments)
94
BioVendor Instruments irisin concentrations
Irisin down-regulates PIAS4 to repress EHMT2 SUMOylation, thereby promoting osteogenic differentiation. MSCs were infected with lentivirus containing oe-NC or oe-PIAS4 with or without irisin together. A Osteogenic differentiation potential was evaluated by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). B Western blotting analysis of protein levels of PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1. (C) Cells were lysed and immunoprecipitated with EHMT2 antibody and then immunoblotted with SUMO2/3 or EHMT2 antibody. MSCs were treated with irisin together with or without <t>irisin</t> <t>inhibitor</t> <t>RGDS.</t> (D) Osteogenic differentiation potential was determined by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). (E) The protein levels of FAK, p-FAK, SKP2, PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1 were assessed by Western blotting. (F) Cell lysates were immunoprecipitated with EHMT2 antibody, followed by immunoblotting with SUMO2/3 or EHMT2 antibody. (G) Ubiquitin level of EHMT2 was analyzed by Co-IP. n = 3. One-way ANOVA (for B, E) was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001
Irisin Concentrations, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant irisin  (MedChemExpress)
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MedChemExpress recombinant irisin
Irisin down-regulates PIAS4 to repress EHMT2 SUMOylation, thereby promoting osteogenic differentiation. MSCs were infected with lentivirus containing oe-NC or oe-PIAS4 with or without irisin together. A Osteogenic differentiation potential was evaluated by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). B Western blotting analysis of protein levels of PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1. (C) Cells were lysed and immunoprecipitated with EHMT2 antibody and then immunoblotted with SUMO2/3 or EHMT2 antibody. MSCs were treated with irisin together with or without <t>irisin</t> <t>inhibitor</t> <t>RGDS.</t> (D) Osteogenic differentiation potential was determined by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). (E) The protein levels of FAK, p-FAK, SKP2, PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1 were assessed by Western blotting. (F) Cell lysates were immunoprecipitated with EHMT2 antibody, followed by immunoblotting with SUMO2/3 or EHMT2 antibody. (G) Ubiquitin level of EHMT2 was analyzed by Co-IP. n = 3. One-way ANOVA (for B, E) was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001
Recombinant Irisin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellular proteins fndc5 irisin  (Proteintech)
93
Proteintech cellular proteins fndc5 irisin
Irisin down-regulates PIAS4 to repress EHMT2 SUMOylation, thereby promoting osteogenic differentiation. MSCs were infected with lentivirus containing oe-NC or oe-PIAS4 with or without irisin together. A Osteogenic differentiation potential was evaluated by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). B Western blotting analysis of protein levels of PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1. (C) Cells were lysed and immunoprecipitated with EHMT2 antibody and then immunoblotted with SUMO2/3 or EHMT2 antibody. MSCs were treated with irisin together with or without <t>irisin</t> <t>inhibitor</t> <t>RGDS.</t> (D) Osteogenic differentiation potential was determined by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). (E) The protein levels of FAK, p-FAK, SKP2, PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1 were assessed by Western blotting. (F) Cell lysates were immunoprecipitated with EHMT2 antibody, followed by immunoblotting with SUMO2/3 or EHMT2 antibody. (G) Ubiquitin level of EHMT2 was analyzed by Co-IP. n = 3. One-way ANOVA (for B, E) was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001
Cellular Proteins Fndc5 Irisin, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kit  (Cusabio)
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Cusabio elisa kit
Irisin down-regulates PIAS4 to repress EHMT2 SUMOylation, thereby promoting osteogenic differentiation. MSCs were infected with lentivirus containing oe-NC or oe-PIAS4 with or without irisin together. A Osteogenic differentiation potential was evaluated by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). B Western blotting analysis of protein levels of PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1. (C) Cells were lysed and immunoprecipitated with EHMT2 antibody and then immunoblotted with SUMO2/3 or EHMT2 antibody. MSCs were treated with irisin together with or without <t>irisin</t> <t>inhibitor</t> <t>RGDS.</t> (D) Osteogenic differentiation potential was determined by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). (E) The protein levels of FAK, p-FAK, SKP2, PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1 were assessed by Western blotting. (F) Cell lysates were immunoprecipitated with EHMT2 antibody, followed by immunoblotting with SUMO2/3 or EHMT2 antibody. (G) Ubiquitin level of EHMT2 was analyzed by Co-IP. n = 3. One-way ANOVA (for B, E) was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001
Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kit - by Bioz Stars, 2026-02
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elisa kit  (BioVendor Instruments)
94
BioVendor Instruments elisa kit
Irisin down-regulates PIAS4 to repress EHMT2 SUMOylation, thereby promoting osteogenic differentiation. MSCs were infected with lentivirus containing oe-NC or oe-PIAS4 with or without irisin together. A Osteogenic differentiation potential was evaluated by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). B Western blotting analysis of protein levels of PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1. (C) Cells were lysed and immunoprecipitated with EHMT2 antibody and then immunoblotted with SUMO2/3 or EHMT2 antibody. MSCs were treated with irisin together with or without <t>irisin</t> <t>inhibitor</t> <t>RGDS.</t> (D) Osteogenic differentiation potential was determined by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). (E) The protein levels of FAK, p-FAK, SKP2, PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1 were assessed by Western blotting. (F) Cell lysates were immunoprecipitated with EHMT2 antibody, followed by immunoblotting with SUMO2/3 or EHMT2 antibody. (G) Ubiquitin level of EHMT2 was analyzed by Co-IP. n = 3. One-way ANOVA (for B, E) was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001
Elisa Kit, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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↓ irisin  (BioVendor Instruments)
94
BioVendor Instruments ↓ irisin
Irisin down-regulates PIAS4 to repress EHMT2 SUMOylation, thereby promoting osteogenic differentiation. MSCs were infected with lentivirus containing oe-NC or oe-PIAS4 with or without irisin together. A Osteogenic differentiation potential was evaluated by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). B Western blotting analysis of protein levels of PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1. (C) Cells were lysed and immunoprecipitated with EHMT2 antibody and then immunoblotted with SUMO2/3 or EHMT2 antibody. MSCs were treated with irisin together with or without <t>irisin</t> <t>inhibitor</t> <t>RGDS.</t> (D) Osteogenic differentiation potential was determined by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). (E) The protein levels of FAK, p-FAK, SKP2, PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1 were assessed by Western blotting. (F) Cell lysates were immunoprecipitated with EHMT2 antibody, followed by immunoblotting with SUMO2/3 or EHMT2 antibody. (G) Ubiquitin level of EHMT2 was analyzed by Co-IP. n = 3. One-way ANOVA (for B, E) was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001
↓ Irisin, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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irisin level  (BioVendor Instruments)
94
BioVendor Instruments irisin level
Irisin down-regulates PIAS4 to repress EHMT2 SUMOylation, thereby promoting osteogenic differentiation. MSCs were infected with lentivirus containing oe-NC or oe-PIAS4 with or without irisin together. A Osteogenic differentiation potential was evaluated by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). B Western blotting analysis of protein levels of PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1. (C) Cells were lysed and immunoprecipitated with EHMT2 antibody and then immunoblotted with SUMO2/3 or EHMT2 antibody. MSCs were treated with irisin together with or without <t>irisin</t> <t>inhibitor</t> <t>RGDS.</t> (D) Osteogenic differentiation potential was determined by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). (E) The protein levels of FAK, p-FAK, SKP2, PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1 were assessed by Western blotting. (F) Cell lysates were immunoprecipitated with EHMT2 antibody, followed by immunoblotting with SUMO2/3 or EHMT2 antibody. (G) Ubiquitin level of EHMT2 was analyzed by Co-IP. n = 3. One-way ANOVA (for B, E) was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001
Irisin Level, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/irisin level/product/BioVendor Instruments
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Irisin down-regulates PIAS4 to repress EHMT2 SUMOylation, thereby promoting osteogenic differentiation. MSCs were infected with lentivirus containing oe-NC or oe-PIAS4 with or without irisin together. A Osteogenic differentiation potential was evaluated by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). B Western blotting analysis of protein levels of PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1. (C) Cells were lysed and immunoprecipitated with EHMT2 antibody and then immunoblotted with SUMO2/3 or EHMT2 antibody. MSCs were treated with irisin together with or without irisin inhibitor RGDS. (D) Osteogenic differentiation potential was determined by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). (E) The protein levels of FAK, p-FAK, SKP2, PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1 were assessed by Western blotting. (F) Cell lysates were immunoprecipitated with EHMT2 antibody, followed by immunoblotting with SUMO2/3 or EHMT2 antibody. (G) Ubiquitin level of EHMT2 was analyzed by Co-IP. n = 3. One-way ANOVA (for B, E) was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: Cell Communication and Signaling : CCS

Article Title: Down-regulation of EHMT2 through irisin-mediated epigenetic modification promotes osteogenesis via promoting DLX3 transcription

doi: 10.1186/s12964-025-02463-x

Figure Lengend Snippet: Irisin down-regulates PIAS4 to repress EHMT2 SUMOylation, thereby promoting osteogenic differentiation. MSCs were infected with lentivirus containing oe-NC or oe-PIAS4 with or without irisin together. A Osteogenic differentiation potential was evaluated by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). B Western blotting analysis of protein levels of PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1. (C) Cells were lysed and immunoprecipitated with EHMT2 antibody and then immunoblotted with SUMO2/3 or EHMT2 antibody. MSCs were treated with irisin together with or without irisin inhibitor RGDS. (D) Osteogenic differentiation potential was determined by ALP and ARS staining, and quantitative analysis was provided (scale bar = 100 μm). (E) The protein levels of FAK, p-FAK, SKP2, PIAS4, EHMT2, DLX3, Runx2, OCN, OPN, and Colla1 were assessed by Western blotting. (F) Cell lysates were immunoprecipitated with EHMT2 antibody, followed by immunoblotting with SUMO2/3 or EHMT2 antibody. (G) Ubiquitin level of EHMT2 was analyzed by Co-IP. n = 3. One-way ANOVA (for B, E) was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: MSCs were treated with 100 ng/mL irisin (MCE, NJ, USA) together with or without irisin inhibitor RGDS (100 nM, MCE) for 48 h.

Techniques: Infection, Staining, Western Blot, Immunoprecipitation, Ubiquitin Proteomics, Co-Immunoprecipitation Assay